Chrysin-Loaded Microemulsion: Formulation Design, Evaluation and Antihyperalgesic Activity in Mice

Chrysin is a bioactive flavonoid found in pollens, passion flowers, honey, royal jelly, and propolis, which commonly used as an ingredient natural food supplements primarily responsible for their pharmacological properties. A transparent chrysin-loaded microemulsion (CS-ME) prepared through ternary phase diagram was evaluated use antihyperalgesic formulation. It formulated with 40% Labrasol® (surfactant), 5% isopropyl myristate (oil phase) 55% water (aqueous classified oil-in-water (O/W) microsized system (74.4 ± 15.8 nm). Its negative Zeta potential (?16.1 1.9 mV) confirmed by polarized light microscopy dynamic scattering analysis. In vitro studies Franz-type static diffusion cells showed that chrysin release from CS-ME followed zero-order kinetics. Oral administration of mice resulted statistically significantly reduction (p < 0.05) carrageenan-induced mechanical hyperalgesia compared to the control group. Treatment also anti-inflammatory activity decreasing TNF-? level 0.01) increasing IL-10 These results suggest proposed microsystem promising vector chrysin, being able improve its capacity modulate inflammatory nociceptive responses.